How does your data fit into the principles of practicing good aseptic technique?Explain

Write up instruction
• No more than 2400 words in total.
• Introduction – no more than 1 side of A4

1. Aseptic technique and personal hygiene
• How clean is your watch, hair, jewellery, coin etc
• Finger dab pre and post washing.
• Nose swab for Staphylococcus spp
• Do not want details from Gram staining

Introduction
• Talk about the relationship between us and microbes
• Importance of aseptic technique

Methods
• Copy the Methods sheet

Results
• Present data in an appropriate way
• Include the class data for nasal carriage of Staphylococci
– 26 out of 158 people (16%) class data
– Present as a percentage of the population sampled
– Do not discuss your data here, just describe it!

Discussion/conclusions
• How does your data fit into the principles of practicing good aseptic technique?
• How does the Staphylococci colonisation data compare with the published text book?

2. Total viable count from a food sample
• Quantification of microbial load from a food sample (either bean sprouts or cheese)

Introduction
• Why do we want to quantify bacteria from food products?
• How do we quantify bacteria?

Methods
• Copy the methods sheet

Results
• Present your data in an appropriate way
• Remember we want to know the concentration of bacteria in the original sample
• Have a set of data for both bean sprouts and cheese

Discussion/conclusions
• How does your data compare with recommended standards?
• Why did we use the quantification method in question instead of others?
• Why is it important to determine a viable count in foods?

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